METHODOLOGY:

Multiplex Real-Time Polymerase Chain Reaction (RT-qPCR)

 

ANALYTE TYPES:

• Viruses 

• Bacteria

• Parasites

• Toxin/Virulance Factor

 

INTERNAL CONTROL:

• Human RNase P gene (Specimen adequacy + extraction control)

 

TARGET CATEGORIES:

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Viruses (6+ targets):
Rotavirus, Adenovirus, Astrovirus, Norovirus GI/GII, Sapovirus

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Bacteria (6+ targets):
Campylobacter spp., Salmonella enterica, Yersinia enterocolitica, Enteroinvasive E. coli (ipaH), Shiga toxin-producing E. coli (stx1/stx2), Enteropathogenic E. coli (eae)

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Parasites (4 targets):
Giardia lamblia, Cryptosporidium spp., Entamoeba histolytica

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Toxin / Virulence Factors (1 target):
Clostridioides difficile toxin B (tcdB)